NextGen Sciences

• Sample types – human and animal biofluids, tissues and primary cells, cell lines and cell-based model systems, FFPE, and plant samples
• Discovery platforms – mass spectrometry-based; GeLC-MS and differential mass spectrometry, multiplex labelled techniques (SILAC) and 2D gel electrophoresis
• Data analysis – performance evaluation, statistical analysis, and multi-variant analysis and visualisation (PCA and hierarchical clustering)

Biomarker assay development and validation

Biomarker assay development and validation is the development of assays that enable the quantitation of proteins in biological samples.

Our biomarker assay development and validation capabilities:

• Development platform – MRM protein assay (LC-MRM/MS)
• Speed – considerably faster to develop than immunoassays
• Multiplex – up to 30 proteins in a single assay
• Quantitation – relative or absolute validated assay
• Specificity – for targeted protein(s) including post-translational modifications and isoforms
• Cross species – enables development of a single assay for multiple species

Biomarker testing

Biomarker testing is the testing of biological samples to monitor proteins using the assays developed.

Our biomarker testing services:

• Testing platform – MRM protein assay (LC-MRM/MS)
• Scale – sample sets in quantities to the 1,000s
• Validation – take biomarkers all the way through to clinical validation

Protein discovery services – mapping protein complexes from immunoprecipitation experiments

Immunoprecipitation (IP) of intact protein complexes involves employing an antibody that targets a known protein member of a larger complex of proteins. By targeting this known member it is possible to pull the entire protein complex out of solution and thereby identify unknown members. Similarly, the use of tagged proteins (e.g tandem affinity purification [TAP], FLAG-tag) can be employed to achieve the same objective. The task for researchers is to then identify the proteins that are pulled down.

NextGen Sciences uses an optimised procedure to identify the maximum number of proteins.

Protein discovery services – mapping and comparing the proteome of cell lines

NextGen Sciences uses an optimised procedure to provide unsurpassed protein identification from complex mixtures.

Regularly in excess of 3,000 proteins will be identified from a cell line with relative quantitation data. This enables cell lines to be compared to determine the relative up or down regulation for observed proteins of interest.

Biomarker project management

Your assigned project director will be your single point of contact for your study. Regular project updates ensure you know exactly how your project is progressing and that you achieve your objectives. We offer flexibility in report requirements and delivery.