Detection of antigen-specific memory B cells for immune monitoring requires their activation, and is commonly accomplished through stimulation with the TLR7/8 agonist R848 and IL-2.
Cellular Technology Limited (CTL) is a global biotechnology company specialized in the development, use, and manufacture of standardized tools for cellular immune assays.
The company manufactures ImmunoSpotTM analyzers and provides consumables for testing, such as its cryopreserved ePBMC. CTL’s laboratory services include but is not limited to cryopreservation of samples, assay development and validation, regulatory complaint testing, and plate scanning and analysis.
CTL is a leading solution provider for immune monitoring needs during drug and vaccine development, supporting both pre-clinical and clinical studies. Its contract laboratory operation has a unique high-throughput immune monitoring capability of cell-mediated immunity (CMI) with emphasis on T and B-cells in a GCP and GLP-compliant facility.
The company’s laboratory services include but are not limited to, separation of PBMC from whole blood and cryopreservation of functional PBMC, assay development and validation, for ELISPOT, killer assays, ELISA, and FACS. Furthermore, CTL manufactures ImmunoSpotTM analyzers for the evaluation of immunological and bioassays (ELISPOT, viral plaque, serum neutralization and cytopathic effect). CTL’s extensive library of cryopreserved characterized PBMC, and a targeted range of consumables complete its solution portfolio.
Cellular Technology Limited
To help researchers get the best possible results from their assays, the company offers a wide range of products and services, including:
- World-class Contract Research in the company’s CLIA-certified, FDA-compliant, GLP Laboratory. The company’s process and test samples using multiple bioassays, also specializing in high-throughput screening and loss-free cryopreservation .
of clinical samples for trials, shipping, and storage and can customize many R&D services to suit your needs.
- High-performance ImmunoSpot® Plate Readers provide objective, fully-automated ELISPOT data analysis, as well as FluoroSpot, cell counting, and plaque and colony counting (including clonogenic assays).
- Extensive Library of Cryopreserved PBMC for selection of individuals or groups with desired HLA type and antigen reactivity. These cells are ideal as reference standards and assay development, qualification, and validation.
- Streamlined ELISPOT Kits combine high-sensitivity with proven, accurate, and consistent results rendering ELISPOT a simple and precise assay.
- Serum-free Culture Media for the standardized freezing, thawing, and testing of PBMC.
- CEF Peptide Pools are the ideal positive control for activating CD8 memory cells.
- Assay Consultation and Hands-on Wet Lab Training allows the company to share over 150 years of cumulative scientific experience in developing and validating ELISPOT analysis for your specific needs.
A significant quest in the field of immune monitoring is the reliable enumeration of antigen-specific T and B-cells to assess the magnitude of immunity and define the class of the immune response by measuring the lymphocytes’ secretory products. Cancer immunotherapy proficiency panels compared to what extent ELISPOT, tetramer staining and intracellular cytokine staining (ICS) would be suited to meet this goal.
CTL offers everything needed for maximized, standardized ELISPOT work:
- Reference PBMC
- Serum-free media
- Scanning and Analysis Services
- Wetlab Training and Workshop
- Test kits
- Analysis platforms
- GLP compliant Contract Research
White blood cells
The company’s extensive library of cryopreserved peripheral blood mononuclear cells, ePBMC®, offers instant access to characterized, standardized, and quality-controlled PBMC in virtually unlimited quantities. These cells, frozen to retain full functionality, can be used as reagents, with no IRBs needed.
Each of the over 500 donors in the ePBMC® library is high-resolution, HLA-typed, and characterized for immune reactivity to dozens of antigens. This library of PBMC makes it possible to select donors with the desired geno or phenotypes and to test large collectives.
An unlimited number of experiments can be run with the same cells, permitting the standardization of the largest variable in cellular assays, the cell material itself.
At CTL, the company believes that progress in human research will benefit from the ease of repeated access to high-quality, characterized PBMC.
Contract Immune Monitoring and Research
CTL has been providing contract laboratory services for government laboratories, major pharmaceutical companies, biotechnology companies, and academic institutions for nearly 20 years. The company has been recognized by the Cancer Vaccine Consortium as its ELISPOT reference laboratory and has been awarded a contract by the US National Institutes of Health (NIH) to validate the ELISPOT assay for pre-clinical and clinical trials. CTL is a member of the Society of Quality Assurance (SQA) and the Regulatory Affairs Professionals Society (RAPS). CTL’s lab has been certified for Cell Viability by the International Society for Biological and Environmental Repositories (ISBER).
Thanks to the company’s in-house expertise and rigorous procedures, CTL conducts all assays in its regulatory compliant laboratory, whether to validate the test methods to satisfy FDA requirements or evaluate specimens for scientific significance. The company also provides high-throughput data acquisition, evaluation, and quality control, allowing us to process large assays within a relatively short amount of time. For new clients, the company can schedule an initial telephone conference to discuss how the company’s products and services can assist you in achieving your goals.
Until now, the freezing and testing of PBMC samples has required the use of serum. Unfortunately, serum contains a plethora of bioactive molecules whose variable concentrations makes every serum batch unique. The need to work with serum has thus been a significant obstacle to reproducible and standardized ex vivo experimentation with primary cells, such as PBMC.
CTL’s media portfolio permits every single step of ex vivo PBMC work to be performed under serum-free conditions: loss-free freezing, thawing, and testing. You can avoid unspecific high background reactivity while supporting specific cellular functions. CTL’s serum-free media offers all these advantages at a cost comparable to serum-containing media.
The evaluation of many bioassays requires accurate recognition of complex optical patterns in test wells. While the expert’s eye is remarkable in judging morphologies, visual evaluation is tedious, does not leave transparent audit trails, and is error-prone. CTL has created a reader platform, Biospot®, that evaluates these assays with the accuracy of an expert’s eye, minus the shortcomings. The Biospot® platform is suitable for:
- Viral Plaque Assays (PRNT, FRNT, PFU)
- Bacterial Colony Counting (SBA, SBT, OPK, OPA)
- Clonogenic Assays, Cancer, Stem Cell CFU
- Genotoxic Assays, Ames Test, MLA
- Microbial Colony Counting, MPN, TVC, MIC
Plasma-derived (pd) or recombinant (r) therapeutic factor VIII proteins (FVIIIs) are infused to arrest/prevent bleeding in patients with hemophilia A (PWHA). However, FVIIIs are neutralized if anti-FVIII-antibodies (inhibitors) develop.
Characterization of the HCMV-Specific CD4 T Cell Responses that Are Associated with Protective Immunity
Read CTL's latest whitepaper about HCMV