Each antibody-antigen interaction has unique characteristics; a protocol that gives good results for one antibody-antigen pair might not be satisfactory for a second antibody, even on the same sample. The interaction of antisera with protein epitopes in Western blot depends on a number of factors, all of which contribute to the final signal / noise ratio.
Knowledge and control of these factors allows for modifications and optimisation of the procedure. Agrisera, a Swedish company that specialises in polyclonal and monoclonal antibody production, has extensive experience and expertise in this area, and can offer Western Blot recommendations, troubleshooting and protocol advice.
To find out more click the ‘Western Blot recommendations’ link at the bottom of Agrisera’s profile, or contact the company directly.