Fluoresentric

• Drug effects on gene expression
• Drug efficacy on biochemical expression
• NAT design (singleplex, duplex, multiplex and internal positive controls)
• NAT development (singleplex, duplex, multiplex and internal positive controls)
• Quality control for NAT reagents (oligonucleotides, buffers and enzymes)
• NAT validation (specificity, sensitivity, LLOD and LLOQ)
• NAT reagent stability studies
• Custom ready-to-use NAT reaction mixes (lyophilized)

Bioanalytical services

Our bioanalytical services include:

• Analytes: genomic DNA, exogenous DNA, total RNA, mRNA, siRNA, biomolecules, pro-drugs, drugs and metabolites
• Matrices: any
• Quantitative real-time PCR, relative gene expression, genotyping services, SNP discovery and target sequence discovery
• Biochemical expression / content

Custom PCR assay – design, optimization and consumables

FI understands the cost pressures in the pharmaceutical environment. We ensure that we develop products that can be manufactured in a timely and reproducible manner using cost-effective materials. Beginning with a concept, the experts at FI can develop ideas into custom tests and products.

FI provides the following:

• Consultation to plan how to achieve maximum data recovery per reaction
• Assay redesign, reoptimization or troubleshooting for existing PCR assays
• Optimization of NAT assays to your performance criteria
• High-throughput NAT assay development
• Quantitative real-time PCR (RT-PCR), and reverse transcription RT-PCR assays
• Genotyping assays in a variety of probe chemistries
• Instrumentation usage support
• Assay reagent development
• Sample preparation protocol development
• Custom packaging including lyophilization

In addition, FI offers gene expression with cognate biochemical quantification to confirm bioactivity of pharmaceutical formulations, clinical-sample collection, and preparation solutions.

Pharmacogenomics – drug sensitivity and resistance profiling

FI has developed a patent-pending process for the detection of drug response markers. Examples include:

• Tuberculosis multiple drug-resistance screening
• Malaria chloroquine resistance screening
• MRSA marker screening
• Human cancer cell resistance to taxanes
• HIV resistance to nucleoside analogues

PCR sample preparation technologies

Our PCR sample preparation technoloiges include:

• High-throughput real-time PCR ready sample preparation kits
• Moderate-throughput real-time PCR ready sample preparation kits
• Clinical sample nucleic acid extraction kits, suitable for point of care testing
• Custom sample preparation kit development