Manufacturer and Supplier of Customer-Defined Oligonucleotides
DNA Technology is a leading manufacturer and supplier of customer-defined oligonucleotides. DNA Technology’s research and development activities are conducted with a particular focus on development of a product line comprised of molecular diagnostic systems.
DNA Technology is a worldwide supplier of PCR-based diagnostic systems, customised molecular services and custom oligonucleotides. The company is ISO 9001:2008-certified.
We offer a range of oligonucleotide primers. ShortPrimers™ are small-scale DNA oligonucleotide syntheses up to 35 bases, delivered dry in microtiter tubes. They can be modified with 5’Amino, 5’HEX, 5’TET and 5’FAM.
ScanPrimers™ are small-scale DNA oligonucleotide syntheses up to 60 bases, delivered re-suspended in water in screw-cap tubes. They can be modified with dInosine, 5’Amino, 5’Biotin, 5’FAM, 5’HEX and 5’TET.
GoldPrimers™ are medium-scale DNA oligonucleotide syntheses up to 75 bases, delivered re-suspended in water in screw-cap tubes. They can be modified with dInosine, 5’Amino, 5’Biotin, 5’Cy5, 5’Cy3, 5’HEX, 5’TET and 5’FAM.
We are experienced in the synthesis of oligonucleotides up to 175 bases. We divide our service into synthesis of up to 75 bases (DNA oligo <76 bases), synthesis of between 76 and 150 bases (DNA oligo 76-150 bases) and synthesis of more than 150 bases (DNA oligo >150).
These oligonucleotides can be modified with a wide range of modifications both in the 5’end, internally and in the 3’end. We have experience in incorporating a wide range of both commercial and customer modifications.
Oligo plates are the perfect format if you need a large number of oligos. The oligos are delivered in a 96-well format and can be used with hand-held pipettes or an automated robot setup.
With the Oligo30Plate™ 96 unmodified oligonucleotides are delivered dry in microtiter plates; oligonucleotides are up to 35 bases long. The Oligo60Plate™ is perfect for making your own oligonucleotide arrays. 96 unmodified or 5’Amino-modified oligonucleotides are delivered dry in microtiter plates; oligonucleotides are up to 75 bases long.
Both the Oligo30Plate and the Oligo60Plate have a guaranteed yield of 4nmol per oligonucleotide.
Customised oligonucleotide modifications
Oligonucleotide modifications are divided into 5’position modifications, 3’position modifications, and internal or 5’position modifications. Internal or 5’position modifications can also be inserted at the 3’end of an oligonucleotide using special procedures. We also have experience in performing customised oligonucleotide modifications – contact us for more information.
Internal or 5’redundancies are made without additional charges; we only charge extra for 3’redundancies. Redundancies or mixed-base positions are indicated using the ambiguity table from the International Union of Biochemistry.
Doped oligonucleotides are described by inserting numbers (1, 2, 3, 4, 5, etc.) in the sequence in the doped positions. Each number may refer to a specific kind of doping.
Purification of synthetic oligonucleotides
We offer purification options to remove the failure sequences and various types of cleaved protection groups naturally generated during the synthesis and modification of synthetic oligonucleotides. These oligonucleotide purification methods are:
- Ethanol precipitated
- Reverse phase high-performance liquid chromatography (RP-HPLC)
- Alkaline RP-HPLC
RNA oligonucleotide synthesis and phosphorothioate oligonucleotides
RNA oligonucleotides can be synthesised by different strategies, which differ by the choice of protection of the 2’hydroxyl group on the ribose. DNA Technology offers 2’O-methyl or 2’O-silyl RNA synthesis.
- O-methyl RNA oligonucleotide synthesis: methyl moiety at the 2’hydroxyl is not removed prior to use resulting in nuclease resistance
- O-silyl RNA oligonucleotide synthesis: the most common strategy to produce ordinary RNA oligonucleotides is by 2’protection with O-Silyl; post-synthesis, the oligonucleotides are deprotected (desilylated) and desalted
- Phosphorothioate DNA and RNA: phosphorothioate oligonucleotides are isoelectronic analogues of natural oligonucleotides in which one of the non-bridging internucleotide oxygen atoms is replaced by a sulphur atom
CE-marked kits for in vitro diagnostics
We manufacture several different CE-marked kits that are sold worldwide, including:
- HemaVision: detection of chromosomal alterations and translocations (CE-marked for in vitro diagnostic use)
- EnteroVision: 5hr detection of enteroviruses
- FluAVision: 5hr detection of influenza A viruses and H5/H7 subtypes